Interactomics

Overview

Syllabus 8

1. Week 1
   • Lecture 1 : Introduction to Interactomics
   • Lecture 2 : An overview of label-free technologies
   • Lecture 3 : An overview of surface plasmon resonance (SPR)
   • Lecture 4 : An overview of surface plasmon resonance imaging (SPRi)
   • Lecture 5 : Basics of SPR: Surface chemistry
2. Week 2
   • Lecture 6 : Basics of SPR: Experimental design
   • Lecture 7 : Protein immobilization for protein-protein interaction studies
   • Lecture 8 : Protein-protein interaction study: Binding analysis
   • Lecture 9 : Protein-protein interaction study: Kinetic analysis
   • Lecture 10 : Protein-small molecule interaction study: Immobilization & binding analysis
3. Week 3
   • Lecture 11 : Protein-small molecule interaction study: Kinetic analysis
   • Lecture 12 : SPR: Interactive Session - I
   • Lecture 13 : SPR: Interactive Session - II
   • Lecture 14 : An overview of ellipsometry and interferometry techniques
   • Lecture 15 : An introduction to BioLayer Interferometry (BLI) and its applications in protein research
4. Week 4
   • Lecture 16 : Kinetic analysis of protein-protein interaction using BLI
   • Lecture 17 : Label-free quantification of proteins using BLI
   • Lecture 18 : Diffraction-based biosensors - I
   • Lecture 19 - Diffraction-based biosensors - II
   • Lecture 20 - Nanotechniques in proteomics - I
   • Lecture 21 - Nanotechniques in proteomics - II
5. Week 5
   • Lecture 22 : High throughputplatforms of interactomics: Protein arrays
   • Lecture 23 : Conventional label based detection techniques for Protein microarrays
   • Lecture 24 : Novel detection techniques for Protein microarrays
   • Lecture 25 : Recombinational cloning and its application for Protein microarrays
   • Lecture 26 : An introduction to Cell-free protein synthesis
6. Week 6
   • Lecture 27 : Cell-free synthesis based protein microarrays: PISA and NAPPA
   • Lecture 28 : Cell-free synthesis based protein microarrays: MIST, DAPA and Halotag Arrays
   • Lecture 29 : Digging deeper into NAPPA: Basic Workflow
   • Lecture 30 : Digging deeper into NAPPA: Surface Chemistry, Printing and Assessment
   • Lecture 31 : Application of cell free expression protein microarrays in biomarker discovery
7. Week 7
   • Lecture 32 : Application of cell-free expression protein microarrays in immunological studies
   • Lecture 33 : Basics of microarray image scanning
   • Lecture 34 : Software for Image scanning and data processing
   • Lecture 35 : Microarray Data Analysis : Part I
   • Lecture 36 : Microarray Data Analysis : Part II
8. Week 8
   • Lecture 37 : Application of protein microarray in biomarker discovery-I
   • Lecture 38 : Application of protein microarray in biomarker discovery-II
   • Lecture 39 : Systems biology and networks
   • Lecture 40 : Challenges in proteomics

Advantages and disadvantages

Some points apply to every course of this kind; see how we rank.

Taught by

• Prof. Sanjeeva SrivastavaIIT Bombay